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Cell Nucleus

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Where does the DNA live?

The answer depends on if the cell is prokaryotic or eukaryotic. The main difference between the two types of cells is the presence of a nucleus. And in eukaryotic cells, DNA lives in the nucleus.

The Nucleus

The nucleus is a membrane-enclosed organelle found in most eukaryotic cells. The nucleus is the largest organelle in the cell and contains most of the cell's genetic information (mitochondria also contain DNA, called mitochondrial DNA, but it makes up just a small percentage of the cell’s overall DNA content). The genetic information, which contains the information for the structure and function of the organism, is found encoded in DNA in the form of genes. A gene is a short segment of DNA that contains information to encode an RNA molecule or a protein strand. DNA in the nucleus is organized in long linear strands that are attached to different proteins. These proteins help the DNA coil up for better storage in the nucleus. Think how a string gets tightly coiled up if you twist one end while holding the other end. These long strands of coiled-up DNA and proteins are called chromosomes. Each chromosome contains many genes. The function of the nucleus is to maintain the integrity of these genes and to control the activities of the cell by regulating gene expression. Gene expression is the process by which the information in a gene is "decoded" by various cell molecules to produce a functional gene product, such as a protein molecule or an RNA molecule.

T he nuclear envelope is a double membrane of the nucleus that encloses the genetic material. It separates the contents of the nucleus from the cytoplasm . The nuclear envelope is made of two lipid bilayers, an inner membrane and an outer membrane. The outer membrane is continuous with the rough endoplasmic reticulum. Many tiny holes called nuclear pores are found in the nuclear envelope. These nuclear pores help to regulate the exchange of materials (such as RNA and proteins) between the nucleus and the cytoplasm.

The eukaryotic cell nucleus. Visible in this diagram are the ribosome-studded double membranes of the nuclear envelope, the DNA (as chromatin), and the nucleolus. Within the cell nucleus is a viscous liquid called nucleoplasm, similar to the cytoplasm found outside the nucleus. The chromatin (which is normally invisible), is visible in this figure only to show that it is spread throughout the nucleus.

Vocabulary

  • chromatin : Form of DNA when it is not coiled into chromosomes.
  • chromosome : Coiled structure made of DNA and proteins; contains sister chromatids; the form of the genetic material of a cell goes during cell division.
  • gene : Unit of DNA that is encoded with the instructions for a single polypeptide.
  • gene expression : The use of a gene to make a mRNA/protein.
  • nuclear envelope : Double membrane of the nucleus; encloses the genetic material.
  • nuclear pore : Tiny hole in the nuclear envelope.
  • nucleolus : Section of the nucleus; site of ribosome assembly.
  • nucleus (plural, nuclei ): Organelle inside eukaryotic cells that contains most of the cell’s DNA; control center of the ce ll.
  • ribosome : Organelle inside all cells where proteins are made; site of protein synthesis.

Summary

  • The nucleus is a membrane-enclosed organelle, found in most eukaryotic cells, which stores the genetic material (DNA).
  • The nucleus is surrounded by a double lipid bilayer, the nuclear envelope, which is embedded with nuclear pores.
  • The nucleolus is inside the nucleus, and is where ribosomes are made.

Practice

Use this resource to answer the questions that follow.

  1. How big is a typical nucleus?
  2. Describe the structure and role of the nuclear envelope.
  3. What is a nuclear pore?
  4. What is chromatin?
  5. What is the difference between nucleoplasm and cytoplasm?
  6. What occurs in the nucleolus?

Review

1. What is the role of the nucleus of a eukaryotic cell?

2. Describe the nuclear membrane.

3. What are nuclear pores?

4. What is the role of the nucleolus?

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