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3.6: Translation of RNA to Protein

Difficulty Level: Basic / At Grade Created by: CK-12
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How does the cell translate a message?

The mRNA is the message sent from the nucleus to the ribosome. Like a foreign language, the genetic code of the mRNA message must then be translated so that the ribosomes make the correct protein. The process of reading the code of a mRNA to make a protein is called translation.


The mRNA, which is transcribed from the DNA in the nucleus, carries the directions for the protein-making process. mRNA tells the ribosome (Figure below) how to create a specific protein.

Ribosomes translate RNA into a protein with a specific amino acid sequence. The tRNA binds and brings to the ribosome the amino acid encoded by the mRNA.

The process of reading the mRNA code in the ribosome to make a protein is called translation (Figure below): the mRNA is translated from the language of nucleic acids (nucleotides) to the language of proteins (amino acids). Sets of three bases, called codons, are read in the ribosome, the organelle responsible for making proteins.

This summary of how genes are expressed shows that DNA is transcribed into RNA, which is translated, in turn, to protein. The one letter code represents amino acids.

The following are the steps involved in translation:

  • mRNA travels to the ribosome from the nucleus.

The following steps occur in the ribosome:

  • The base code in the mRNA determines the order of the amino acids in the protein. The genetic code in mRNA is read in “words” of three letters (triplets), called codons. There are 20 amino acids used to make proteins, and different codons code for different amino acids. For example, GGU codes for the amino acid glycine, while GUC codes for valine.
  • tRNA reads the mRNA code and brings a specific amino acid to attach to the growing chain of amino acids. The anticodon on the tRNA binds to the codon on the mRNA. Each tRNA carries only one type of amino acid and only recognizes one specific codon.
  • tRNA is released from the amino acid.
  • Three codons, UGA, UAA, and UAG, indicate that the protein should stop adding amino acids. They are called stop codons and do not code for an amino acid. Once tRNA comes to a stop codon, the protein is set free from the ribosome.

The following chart (Figure below) is used to determine which amino acids correspond to which codons.

This chart shows the genetic code used by all organisms. For example, an RNA codon reading GUU would encode for a valine (Val) according to this chart. Start at the center for the first base of the three base codon, and work your way out. Notice that more than one codon may encode for a single amino acid. For example, glycine (Gly) is encoded by a GGG, GGA, GGC, and GGU.


  • anticodon: Sequence on a tRNA molecule that specifically binds to a codon; ensures the proper amino acid is brought to the ribosome.
  • codon: Triplet (3) of bases in the mRNA that codes for a specific amino acid.
  • ribosome: Organelle responsible for making proteins.
  • stop codon: One of three codons that does not encode for an amino acid; signifies the end of translation.
  • translation: Process of reading the mRNA code in the ribosome to make a protein.


  • Translation is the process of reading the mRNA code in the ribosome to make a protein.
  • Sets of three bases on the mRNA, called codons, are read in order to select the correct amino acid for building a protein.


Use the resources below to answer the following questions.

  1. What reads the sequence of the mRNA? What are three nucleotides that code of an amino acid called?
  2. What brings amino acids to the translation site? Where does it find these amino acids?
  3. What is an anticodon? Where are they found? What is their function?
  4. About how many amino acids are present in your average protein?
  5. How many ribosomes read a single mRNA molecule at the same time? How is this beneficial to the organism?

Go to this site to make a protein. Practice and see how fast you can transcribe DNA and translate mRNA.

  1. What is the start code for translating an mRNA molecule?
  2. How many stop codes are there from translating mRNA? What are they?
  3. How many amino acids are used to make proteins?


  1. What is translation?
  2. What would happen if the stop codon was mutated to encode for another amino acid?
  3. Given the DNA sequence, ATGTTAGCCTTA, what is the mRNA sequence? What is the amino acid sequence?

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Nov 29, 2012

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Apr 11, 2014
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\n Front Matter\n


\n Foreword\n


\n Preface\n


\n The study of life is one of the fundamental fields of the middle school curriculum. The CK-12 Life Science Concepts presents life science as a set of 12 concepts, with each concept centered around a specific category, such as cell biology or human biology. Each concept is comprised of a series of lessons, with each lesson focusing on one specific topic. The complete Life Science Concepts is comprised of over 250 lessons. These lessons present life science to the middle school student in an easy to follow, easily understandable format.\n


\n Chapters\n


\n \"\"\n

\n \n

\n Back Matter\n

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Original text