How can we see the details of bacteria?
With the naked eye, bacteria just look like a slimy smear on a petri dish. How can we study them in more detail? The invention of the microscope has allowed us to see bacteria, cells, and other things too small to be seen with the naked eye.
Microscopes, tools that you may get to use in your class, are some of the most important tools in biology (Figure below). A microscope is a tool used to make things that are too small to be seen by the human eye look bigger. Microscopy is the study of small objects using microscopes. Look at your fingertips. Before microscopes were invented in 1595, the smallest things you could see on yourself were the tiny lines in your skin. But what else is hidden in your skin?
Basic light microscopes opened up a new world to curious people.
Invention of the Microscope
Over four hundred years ago, two Dutch spectacle makers, Zaccharias Janssen and his son Hans, were experimenting with several lenses in a tube. They discovered that nearby objects appeared greatly enlarged, or magnified. This was the forerunner of the compound microscope and of the telescope.
In 1665, Robert Hooke, an English natural scientist, used a microscope to zoom in on a piece of cork - the stuff that makes up the stoppers in wine bottles, which is made from tree bark. Inside of cork, he discovered tiny structures, which he called cells. It turns out that cells are the smallest structural unit of living organisms. This finding eventually led to the development of the theory that all living things are made of cells. Without microscopes, this discovery would not have been possible, and the cell theory would not have been developed.
Hooke's discovery of the cell set the stage for other scientists to discover other types of organisms. After Hooke, the "father of microscopy," Dutch scientist Antoine van Leeuwenhoek (Figure below) taught himself to make one of the first microscopes. In one of his early experiments, van Leeuwenhoek took a sample of scum from his own teeth and used his microscope to discover bacteria, the smallest living organism on the planet. Using microscopes, van Leeuwenhoek also discovered one-celled protists and sperm cells.
Today, microscopes are used by all types of scientists, including cell biologists, microbiologists, virologists, forensic scientists, entomologists, taxonomists, and many other types.
Antoine van Leeuwenhoek, a Dutch cloth merchant with a passion for microscopy.
Types of Microscopes
Some modern microscopes use light, as Hooke's and van Leeuwenhoek's did. Others may use electron beams or sound waves. Researchers now use these four types of microscopes:
- Light microscopes allow biologists to see small details of a specimen. Most of the microscopes used in schools and laboratories are light microscopes. Light microscopes use lenses, typically made of glass or plastic, to focus light either into the eye, a camera, or some other light detector. The most powerful light microscopes can make images up to 2,000 times larger.
- Transmission electron microscopes (TEM) focus a beam of electrons through an object and can make an image up to two million times bigger, with a very clear image.
- Scanning electron microscopes (SEM) allow scientists to find the shape and surface texture of extremely small objects, including a paperclip, a bedbug, or even an atom. These microscopes slide a beam of electrons across the surface of a specimen, producing detailed maps of the surface of objects. Magnification in a SEM can be controlled over a range from about 10 to 500,000 times.
- Scanning acoustic microscopes use sound waves to scan a specimen. These microscopes are useful in biology and medical research.
A scanning electron microscope.
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- A microscope is a tool used to make things that are too small to be seen by the naked eye look bigger.
- Types of microscopes include light microscopes, transmission electron microscopes (TEM), scanning electron microscopes (SEM), and scanning acoustic microscopes.
Use the resources below to answer the questions that follow.
Explore More I
- Using a microscope at http://www.youtube.com/watch?v=bGBgABLEV4g (4:01)
- How should you carry a compound optical microscope?
- What procedure should you use when seeking to use the most powerful optical lenses?
Explore More II
- Dissecting Microscope at http://www.youtube.com/watch?v=JoqOwzLyMIA (5:05)
- What light sources can you use with a dissecting microscope?
- Why is it important to have a fixed ocular lens and an adjustable ocular lens?
- What happens to your field of view as you increase magnification? Can you explain why this happens?
Explore More III
- Structure and Function of the Electron Microscope at http://www.youtube.com/watch?v=fToTFjwUc5M (1:49)
- How does an electron microscope differ from a light microscope? List all the differences you can think of.
- How should you carry an electron microscope?
Explore More IV
- Scanning Electron Microscope at http://www.youtube.com/watch?v=lrXMIghANbg (5:04)
- How is the electron beam focused?
- What part of a specimen does a scanning electron microscope look at?
- Why is it important that a specimen for an electron microscope be placed in a vacuum? Why is this step unnecessary for a light microscope?
- What is the purpose of a microscope?
- What were the findings of Hooke and van Leeuwenhoek?
- What are the differences between a light microscope and a scanning electron microscope?