Flexi Says: In prophase I of meiosis I, homologous chromosomes pair up and crossing-over occurs during this phase. In prophase II of meiosis II, homologous chromosomes do not form pairs in this way. In metaphase I of meiosis I, spindle fibers attach to the paired homologous chromosomes. The paired chromosomes line up along the equator (middle) of the cell. In metaphase II of meiosis II, it is sister chromatids that line up along the equator of the cell. In anaphase I of meiosis I, spindle fibers shorten, and the chromosomes of each homologous pair start to separate from each other. One chromosome of each pair moves toward one pole of the cell, and the other chromosome moves toward the opposite pole. In anaphase II of meiosis II, sister chromatids separate and move to opposite poles. In telophase I and cytokinesis of meiosis I, the cytoplasm of the cell divides, and two haploid daughter cells result. The daughter cells each have a random assortment of chromosomes, with one from each homologous pair. Both daughter cells go on to meiosis II. The DNA does not replicate between meiosis I and meiosis II. In telophase II and cytokinesis of meiosis II, the cytoplasm of each cell divides, and four haploid cells result. Each cell has a unique combination of chromosomes.