Flexi Says: Biuret solution is an indicator that is used to test samples for proteins. Biuret is a compound that forms when urea is heated. This compound is mixed with potassium hydroxide (KOH) and copper (II) sulfate (CuSO4) to form a blue solution that is used as an indicator. The blue solution turns violet in the presence of proteins, and changes to pink when combined with short-chain polypeptides.